cloning, molecular analysis and epitopics prediction of a new chaperone groel brucella melitensis antigen

Authors

mohammad hadi sekhavati faculty of agriculture, ferdowsi university of mashhad, mashhad, iran

reza majidzadeh heravi faculty of agriculture, ferdowsi university of mashhad, mashhad, iran

mojtaba tahmoorespur faculty of agriculture, ferdowsi university of mashhad, mashhad, iran

soheil yousefi faculty of agriculture, ferdowsi university of mashhad, mashhad, iran

abstract

objective(s):brucellosis is a well-known domestic animal infectious disease, which is caused by brucella bacterium. groel antigen increases brucella survival and is one of the major antigens that stimulates the immune system. hence, the objective of the present study was cloning and bioinformatics analysis of groel gene. materials and methods: the full-length open reading frame of this gene was amplified by specific primers and cloned into ptz57r/t vector. also, the sequence of this gene in the brucella melitensis strain rev 1 was submitted to the ncbi gene bank for the first time. several prediction software applications were also used to predict b and t-cell epitopes, secondary and tertiary structures, antigenicity ability and enzymatic degradation sites. the used software applications validated experimental results. results: the results of phylogenetic analysis showed that the groel sequence had near homology with other species instead of other brucella spp. the bioinformatics tools used in the current study were validated by the results of four different experimental epitope predictions. bioinformatics analysis identified eight b and seven t-cell epitopes. conclusion: according to the antigenic ability and proteasomal cleavage sites, four (150-160, 270-285,351-361 and 385-395) common epitopes were predicted for groel gene. bioinformatics analysis showed that these regions had proper epitope characterization and so may be useful for stimulation of cell-mediated and humoral immunity system.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Cloning, molecular analysis and epitopics prediction of a new chaperone GroEL Brucella melitensis antigen

Objective(s):Brucellosis is a well-known domestic animal infectious disease, which is caused by Brucella bacterium. GroEL antigen increases Brucella survival and is one of the major antigens that stimulates the immune system. Hence, the objective of the present study was cloning and bioinformatics analysis of GroEL gene. Materials and Methods: The full-length open reading frame of this gene was...

full text

Cloning, molecular analysis and epitopics prediction of a new chaperone GroEL Brucella melitensis antigen

OBJECTIVES Brucellosis is a well-known domestic animal infectious disease, which is caused by Brucella bacterium. GroEL antigen increases Brucella survival and is one of the major antigens that stimulates the immune system. Hence, the objective of the present study was cloning and bioinformatics analysis of GroEL gene. MATERIALS AND METHODS The full-length open reading frame of this gene was ...

full text

In silico cloning and bioinformatics study of Brucella melitensis Omp31 antigen in different mammalian expression vectors

Brucella melitensis, as a pathogenic gram-negative intracellular bacterium, causes brucellosis in animals and humans. According to literature, the B. melitensis outer membrane protein 31 (Omp31) is considered as an important vaccine candidate against brucellosis. The aim of the current study was to compare three different expression constructs containing B. melitensis Omp31 antigen using bioinf...

full text

Cloning and molecular characterization of Omp31 gene from Brucella melitensis Rev 1 strain

Brucellosis, caused by the genus Brucella bacterium, is a well-known infection among domestic animals. Considering the serious economic and medical consequences of this infection, various preventive efforts have been made through using recombinant vaccines, based on outer membrane protein (OMP) antigens of Brucella species. The objective of the present study was to clone, analyze the sequence, ...

full text

cloning, expression and molecular analysis of iranian brucella melitensis omp25 gene for designing a subunit vaccine

brucellosis is a well-known domestic animal infectious disease, which is caused by brucella bacterium. the outer membrane protein 25 kda (omp25) gene plays an important role in simulating of tnf-α, ifn-α, macrophage, and cytokines cells. in the current study molecular cloning and expression analysis of omp25 gene for designing a subunit vaccine against brucella was investigated. amplifying the ...

full text

Isolation, cloning and expression of the Brucella melitensis Omp31 gene

Brucellosis is a zoonotic disease transmitted to humans either from animals or from their products.Although brucellosis can be found worldwide, the Mediterranean Basin, South and Central America, EasternEurope, Asia, Africa, the Caribbean, and the Middle East have currently been listed as high-risk regions. Thegenus Brucella is classified in at least nine species. Brucella melitensis is the glo...

full text

My Resources

Save resource for easier access later


Journal title:
iranian journal of basic medical sciences

جلد ۱۸، شماره ۵، صفحات ۴۹۹-۵۰۵

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023